Sarkar, SN and Sikdar , SK (1994) High level stable expression of rat brain type IIA sodium channel ?-subunit in CHO cells. In: Current science (Bangalore), 67 (3). 196 -199 .
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The neuronal sodium channels are responsible for the rising phase of action potential and are composed of three subunits, of which the alpha-subunit has been shown to be adequate for most of its functional properties. We have stably expressed the rat brain type IIA sodium channel alpha-subunit in CHO cell tine using a CMV promoter-based vector. The expression was confirmed by detecting a 6.5 kb RNA corresponding to sodium channel alpha-subunit using Northern hybridization. The cells stably expressing the alpha-subunit, yield isolated sodium currents of amplitudes greater than 4nA when studied in whole-cell configuration of the patch-clamp technique. The sodium currents are characterized by activation and inactivation properties similar to neuronal sodium channels, and are blocked by the voltage gated sodium channel blocker tetrodotoxin (TTX).
|Item Type:||Editorials/Short Communications|
|Additional Information:||Copyright of this article belongs to Indian academy of sciences.|
|Department/Centre:||Division of Biological Sciences > Molecular Biophysics Unit|
|Date Deposited:||01 Apr 2011 05:14|
|Last Modified:||01 Apr 2011 05:14|
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