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Intracellular free calcium level and its response to cAMP stimulation in developing Dictyostelium cells transformed with jellyfish apoaequorin CDNA

Saran, S and Nakao, H and Tasaka, M and Iida, H and Tsuji, FI and Nanjundiah, V and Takeuchi, I (1994) Intracellular free calcium level and its response to cAMP stimulation in developing Dictyostelium cells transformed with jellyfish apoaequorin CDNA. In: FEBS letters, 337 (1). pp. 43-47.

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Official URL: http://dx.doi.org/10.1016/0014-5793(94)80626-8

Abstract

A new method is described for measuring intracellular free calcium concentrations, [(Ca2+)(i)], in the cells of Dictyostelium discoideum transformed with apoaequorin cDNA of the jellyfish, Aequorea victoria. Aequorin, a calcium-specific indicator, was regenerated in vivo from apoaequorin produced in the cells by incubation with coelenterazine. The results showed that [(Ca2+)(i)] in developing cells markedly increases at the aggregation stage and again at the culmination stage after a temporary drop at the migration stage. Except for the vegetative stage, the cells al all stages of development exhibit a sharp transient increase in [(Ca2+)(i)] upon stimulation with a cAMP (50 nM) pulse, high responses being observed at the migration and culmination stages. Separated prestalk cells of migrating slugs contain more than twice as much [(Ca2+)(i)] and show three times as large a response to cAMP stimulation as prespore cells.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Elsevier Science.
Keywords: Dictyostelium discoideum; Aequorin; Cyclic AMP; Calcium; Cell differentiation
Department/Centre: Others
Date Deposited: 24 May 2011 08:04
Last Modified: 24 May 2011 08:04
URI: http://eprints.iisc.ernet.in/id/eprint/36558

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