Structural proteins of mycobacteriophage I3: cloning, expression and sequence analysis of a gene encoding a 70-kDa structural protein

Ramesh, Gundmi R and Gopinathan, Karumathil P (1994) Structural proteins of mycobacteriophage I3: cloning, expression and sequence analysis of a gene encoding a 70-kDa structural protein. In: Gene, 143 (1). pp. 95-100.

PDF Structural_proteins.pdf - Published Version Restricted to Registered users only Download (871Kb) | Request a copy

Abstract

The structural proteins of mycobacteriophage I3 have been analysed by sodium dodecyl sulfate-polyacrylamide-gel electrophoresis (SDS-PAGE), radioiodination and immunoblotting. Based on their abundance the 34- and 70-kDa bands appeared to represent the major structural proteins. Successful cloning and expression of the 70-kDa protein-encoding gene of phage I3 in Escherichia coli and its complete nucleotide sequence determination have been accomplished, A second (partial) open reading frame following the stop codon for the 70-kDa protein was also identified within the cloned fragment. The deduced amino-acid sequence of the 70-kDa protein and the codon usage patterns indicated the preponderance of codons, as predicted from the high G+C content of the genomic DNA of phage I3.

Item Type:	Editorials/Short Communications
Additional Information:	Copyright of this article belongs to Elsevier science.
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology Division of Biological Sciences > Molecular Reproduction, Development & Genetics (formed by the merger of DBGL and CRBME)
Date Deposited:	13 Apr 2011 07:17
Last Modified:	24 Oct 2011 05:17
URI:	http://eprints.iisc.ernet.in/id/eprint/36836

Actions (login required)

View Item