ePrints@IIScePrints@IISc Home | About | Browse | Latest Additions | Advanced Search | Contact | Help

A pathway for biodegradation of 1-naphthoic acid by Pseudomonas maltophilia CSV89

Phale, Prashant S and Mahajan , Milind C (1995) A pathway for biodegradation of 1-naphthoic acid by Pseudomonas maltophilia CSV89. In: Archives of Microbiology, 163 (1). pp. 42-47.

[img] PDF
A_pathway.pdf - Published Version
Restricted to Registered users only

Download (600Kb) | Request a copy
Official URL: http://www.springerlink.com/content/p26128p238j645...

Abstract

Pseudomonas maltophilia CSV89, a bacterium isolated from soil in our laboratory, grows on 1-naphthoic acid as the sole source of carbon and energy. To elucidate the pathway for degradation of 1-naphthoic acid, the metabolites were isolated from spent medium, purified by TLC, and characterized by gas chromatography-mass spectrometry. The involvement of various metabolites as intermediates in the pathway was established by demonstrating relevant enzyme activities in cell-free extracts, oxygen uptake and transformation of metabolites by the whole cells. The results obtained from such studies suggest that the degradation of 1-naphthoic acid is initiated by double hydroxylation of the aromatic ring adjacent to the one bearing the carboxyl group, resulting in the formation of 1,2-dihydroxy-8-carboxynaphthalene. The resultant diol was oxidized via 3-formyl salicylate, 2-hydroxyisophthalate, salicylate and catechol to TCA cycle intermediates.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Springer.
Keywords: Pseudomonas;1-Naphthoic acid;Hydrocarbon degradation; Hydroxylation;Ring cleavage
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 26 Apr 2011 06:19
Last Modified: 26 Apr 2011 06:19
URI: http://eprints.iisc.ernet.in/id/eprint/36920

Actions (login required)

View Item View Item