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Antigenic determinants at the carboxy terminus of chicken egg white riboflavin carrier protein (RCP): Epitope mapping and antibody-mediated pregnancy curtailment in rodents

Koshy, Beena T and Karande, Anjali A and Adiga, Radhakantha P (1996) Antigenic determinants at the carboxy terminus of chicken egg white riboflavin carrier protein (RCP): Epitope mapping and antibody-mediated pregnancy curtailment in rodents. In: Vaccine, 14 (4). 307-312 .

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Official URL: http://dx.doi.org/10.1016/0264-410X(95)00199-B

Abstract

The epitopic core sequences recognized by three monoclonal antibodies raised to chicken riboflavin carrier protein (RCP) were mapped to the C-terminal tail-end of the protein using the pepscan method A 21-residue synthetic peptide corresponding to residues 200-219 of the protein and comprising the regions corresponding to the antibodies was synthesized. Administration of polyclonal antibodies specific to this peptide led to termination of early pregnancy in mice. Also, active immunization of rats with the peptide-purified protein derivative conjugate inhibited establishment of pregnancy. These results demonstrate the functional importance of the C-terminal 200-219 region of chicken RCP. Copyright (C) 1996 Elsevier Science Ltd.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Elsevier science.
Keywords: Chicken riboflavin carrier protein;epitopes;antipeptide antibodies;inhibition of pregnancy establishment
Department/Centre: Division of Biological Sciences > Biochemistry
Division of Biological Sciences > Molecular Reproduction, Development & Genetics (formed by the merger of DBGL and CRBME)
Date Deposited: 10 May 2011 06:22
Last Modified: 20 Oct 2011 07:26
URI: http://eprints.iisc.ernet.in/id/eprint/37029

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