Reddy, Vijay Sai and Rao, VSR (1992) Modes of binding of α(1–2) linked manno-oligosaccharides to concanavalin A. In: International Journal of Biological Macromolecules, 14 (4). pp. 185-192.
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Three-dimensional structures of the complexes of concanavalin A (ConA) with alpha(1-2) linked mannobiose, triose and tetraose have been generated with the X-ray crystal structure data on native ConA using the CCEM (contact criteria and energy minimization) method. All the constituting mannose residues of the oligosaccharide can reach the primary binding site of ConA (where methyl-alpha-D-mannopyranose binds). However, in all the energetically favoured complexes, either the non-reducing end or middle mannose residues of the oligosaccharide occupy the primary binding site. The middle mannose residues have marginally higher preference over the non-reducing end residue. The sugar binding site of ConA is extended and accommodates at least three alpha(1-2) linked mannose residues. Based on the present calculations two mechanisms have been proposed for the binding of alpha(1-2) linked mannotriose and tetraose to ConA.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to Elsevier science.|
|Keywords:||Concanavalin A;(1-2) linked manno-oligosaccharides;three-dimensional structure;conformational energy; energy minimization.|
|Department/Centre:||Division of Biological Sciences > Molecular Biophysics Unit|
|Date Deposited:||05 May 2011 06:02|
|Last Modified:||05 May 2011 06:02|
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