Subramanian, S and Adiga, PR (1996) Hormonal modulation of secretion of immunoreactive riboflavin carrier protein by adult rat Leydig cells in vitro. In: Indian Journal of Biochemistry & Biophysics, 33 (4). 274-280 .Full text not available from this repository.
Adult rat Leydig cells in culture synthesize and secrete riboflavin carrier protein (RCP) as demonstrated by [S-35]-methionine incorporation into newly synthesized proteins followed by immunoprecipitation as well as specific radioimmunoassay. LH stimulates the secretion of RCP 4-fold which could be inhibited upto 75% by an aromatase inhibitor. 8-bromo-cyclic AMP and cholera toxin could mimic the LH stimulated secretion of the carrier protein. The extent of stimulation of RCP secretion brought about by exogenous estradiol-17 beta is comparable to that of LH. The antiestrogen tamoxifen, when added along with either LH or estrogen, inhibited the stimulated levels significantly. These results show that the estrogen-inducible riboflavin carrier is secreted by Leydig cells under positive regulation of LH.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to National institute of science communication and information resources.|
|Department/Centre:||Division of Biological Sciences > Biochemistry
|Date Deposited:||28 Apr 2011 09:54|
|Last Modified:||28 Apr 2011 09:54|
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