Abhilash, Joseph and Geethanandan, K and Bharath, SR and Sadasivan, C and Haridas, M (2011) Crystallization and preliminary X-ray diffraction analysis of a galactose-specific lectin from the seeds of Butea monosperma. In: Acta Crystallographica Section F, 67 (Part 4). pp. 524-526.
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The galactose-specific lectin from the seeds of Butea monosperma has been crystallized by the hanging-drop vapour-diffusion technique. The crystals belonged to space group P1, with unit-cell parameters a = 78.45, b = 78.91, c = 101.85 A, alpha = 74.30, beta = 76.65, gamma = 86.88 degrees. X-ray diffraction data were collected to a resolution of 2.44 A under cryoconditions (100 K) using a MAR image-plate detector system mounted on a rotating-anode X-ray generator. Molecular-replacement calculations carried out using the coordinates of several structures of legume lectins as search models indicate that the galactose-specific lectin from B. monosperma forms an octamer.
|Item Type:||Journal Article|
|Keywords:||galactose-specific lectin;Butea monosperma|
|Department/Centre:||Division of Biological Sciences > Molecular Biophysics Unit|
|Date Deposited:||20 May 2011 10:24|
|Last Modified:||20 May 2011 10:24|
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