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In vitro micropropagation of scented geranium (Pelargonium graveolens L. Her. ex Ait: syn P. roseum willd)

Satyakala, G and Rao, M. Muralidhar and Sita, G. Lakshmi (1995) In vitro micropropagation of scented geranium (Pelargonium graveolens L. Her. ex Ait: syn P. roseum willd). In: Current Science (Bangalore), 68 (7). pp. 762-765.

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Abstract

The objective of this study was to develop a rapid and efficient system for regenerating shoots from nodal explants of scented geranium (Pelargonium graveolens L. Her. ex Ait: syn. P. roseum willd). Single node stem explants were inoculated in MS media containing different combinations of 6-benzylaminopurine (BAP) with indole-3-acetic acid (IAA) or naphthalene acetic acid (NAA) (0, 0.5, 1.0, 2.0 mg/l) in a 4x4 factorial experiment. Multiple shoots were induced in media supplemented with BAP and IAA, Maximum number of shoots (56 per explant) were observed in the medium containing BAP and IAA at 1 mg/l each, 30 days after inoculation. Micro shoots were subcultured once in every four weeks. Adventitious shoots were induced from in vitro grown leaves and petioles. Several regenerated shoots were rooted on MS half-strength medium supplemented with 0.5 mg/l indole-3-butyric acid (IBA) and the plantlets were hardened in the growth chamber. This micropropagation system could be used for rapid and large-scale production of scented geranium.

Item Type: Editorials/Short Communications
Additional Information: Copyright of this article belongs to Indian Academy of Sciences.
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 04 Aug 2011 09:01
Last Modified: 04 Aug 2011 09:01
URI: http://eprints.iisc.ernet.in/id/eprint/38051

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