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Isolation of an 800 kb contiguous DNA fragment encompassing a 3.5-cM region of chromosome 1 in Arabidopsis using YAC clones

Vijayraghavan, U and Siddiqi, I and Meyerowitz, E (1995) Isolation of an 800 kb contiguous DNA fragment encompassing a 3.5-cM region of chromosome 1 in Arabidopsis using YAC clones. In: Genome / Génome, 38 (4). pp. 817-823.

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Official URL: http://www.nrcresearchpress.com/doi/abs/10.1139/g9...

Abstract

The apetalal mutation of Arabidopsis affects floral meristem identity and the development of sepal and petal primordia of the flower. We mapped the available RFLP markers on chromosome 1 that are in the general vicinity of apetalal on a fine structure map and then chose the closest RFLP as a starting point for contiguous DNA (contig) generation. We report here a contig of about 800 kilobases (kb) that spans a 3.5 cM region of chromosome 1. We used genomic libraries of Arabidopsis prepared in yeast artificial chromosome (YAC) vectors and the detailed characterization of 19 YACs is reported. RFLPs displayed by the end fragments from the walk were mapped to align and correlate the genetic and physical maps for this region of chromosome 1. In this segment of the genome, 1 cM corresponds to a little over 200 kb of physical distance.

Item Type: Editorials/Short Communications
Additional Information: Copyright of this article belongs to NRC Research Press.
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 31 May 2011 05:38
Last Modified: 31 May 2011 05:38
URI: http://eprints.iisc.ernet.in/id/eprint/38053

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