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Primary structure of a cytotoxin-like basic protein from Naja naja naja (Indian Cobra) venom

Babu, AS and Puri, KD and Gowda, TV (1995) Primary structure of a cytotoxin-like basic protein from Naja naja naja (Indian Cobra) venom. In: International Journal of Peptide & Protein Research, 46 (1). pp. 69-72.

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Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1399-...

Abstract

The complete amino acid sequence of a cytotoxin-like basic protein (CLBP) from the venom of Naja naja naja (Indian Cobra) was determined by manual degradation using a 4-dimethylaminoazobenzene-4'-isothiocyanate double-coupling method. Peptide fragments obtained by chemical cleavage with cyanogen bromide and enzymic cleavages with trypsin and Staphylococcus aureus proteases for sequence analysis were purified by reversed-phase chromatography. The total number of amino acid residues was 61, with leucine as the C-terminal residue. (C) Munksgaard 1995.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Munksgaard Int Publ ltd.
Keywords: amino acid sequence;cytotoxin-like basic protein;manual degradation;structure-function relationship
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 02 Jun 2011 08:49
Last Modified: 02 Jun 2011 08:49
URI: http://eprints.iisc.ernet.in/id/eprint/38151

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