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In vitro regeneration from immature cotyledon explant and protein profile changes during organogenesis in groundnut (Arachis hypogaea L.)

Venkatachalam, P and Geetha, N and Jayabalan, N (1997) In vitro regeneration from immature cotyledon explant and protein profile changes during organogenesis in groundnut (Arachis hypogaea L.). In: Tropical Agriculture, 74 (2). pp. 140-145.

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Official URL: http://agris.fao.org/agris-search/search/display.d...

Abstract

Complete plants were regenerated from in vitro cultured immature cotyledon segments of groundnut (Arachis hypogaea L. cv. TMV-7) by organogenesis. Callus cultures were best Initiated from immature cotyledon segments on MS (Murashige and Skoog) salts containing B5 vitamins supplemented with indole-3-acetic acid (IAA) and alpha -naphthalene acetic acid (NAA; 4.0 mg L-1) and kinetin (KIN; 0.5 L-1). Calluses were transferred to a medium containing KIN (2.0 mg L-1) and IAA and NAA (0.5 mg L-1) for shoot Initiation. The regenerated shoots were transferred to a medium containing Indole-3-butyric acid (IBA; 2.0 mg L-1) and KIN (0.2 mg L-1) for developing roots. In vitro produced plantlets developed sucessfully, matured, and set seed. The protein profiles [sodium dodecyl sulphate - polyacrylamide gel electrophoresis (SDS-PAGE)] of callus, callus with shoot, and callus with shoot and root showed differences.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to TROPICAL AGRICULTURE.
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 18 Oct 2011 04:37
Last Modified: 18 Oct 2011 04:37
URI: http://eprints.iisc.ernet.in/id/eprint/38277

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