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Isolation of transforming growth factor-beta 2 cDNA from a fish, Cyprinus carpio by RT-PCR

Sumathy, K and Desai, Kartiki V and Kondaiah, Paturu (1997) Isolation of transforming growth factor-beta 2 cDNA from a fish, Cyprinus carpio by RT-PCR. In: Gene, 191 (1). pp. 103-107.

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Official URL: http://dx.doi.org/10.1016/S0378-1119(97)00048-6

Abstract

Transforming Growth Factors-beta (TGF-beta s) have been described in many vertebrate species of amphibians, aves and mammals. In this report we demonstrate the presence of TGF-beta 2 in pisces. TGF-beta 2 has been cloned from a fish, Cyrinus carpio, by RT-PCR using degenerate oligonucleotide primers. Sequence analysis of the amplified product and alignment of the deduced amino acid sequence with the human TGF-beta 2 amino acid sequence revealed 81% and 93% identity in the precursor and the mature regions, respectively. The northern blot analysis of fish heart RNA shows a major messenger RNA species of about 8.0 kb and two messages of very low abundance of about 5.0 kb and 4.0 kb. The identification of TGF-beta 2 isoform in Pisces and it's high degree of homology with the mammalian isoform suggests that among all TGF-beta isoforms, TGF-beta 2 is the most conserved during evolution. (C) 1997 Elsevier Science B.V.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Elsevier Science.
Keywords: Pisces;Heart;DNA sequence;Degenerate PCR
Department/Centre: Division of Biological Sciences > Molecular Reproduction, Development & Genetics (formed by the merger of DBGL and CRBME)
Date Deposited: 29 Aug 2011 10:24
Last Modified: 02 Nov 2011 07:50
URI: http://eprints.iisc.ernet.in/id/eprint/38280

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