Raghavendra, Nidhanapati K and Rao, Desirazu N (2005) Exogenous AdoMet and its analogue sinefungin differentially influence DNA cleavage by R.EcoP151 - Usefulness in SAGE. In: Biochemical and Biophysical Research Communications, 334 (3). pp. 803-811.
While it has been demonstrated that AdoMet is required for DNA cleavage by Type III restriction enzymes, here we show that in the presence of exogenous AdoMet, the head-to-head oriented recognition sites are cleaved only on a supercoiled DNA. On a linear DNA. exogenous AdoMet strongly drives methylation while inhibiting cleavage reaction.Strikingly, AdoMet analogue sine fungin results in cleavage at all recognition sites irrespective of the topology of DNA. The cleavage reaction in the presence of sinefung in is ATP dependent The site of cleavage is comparable with that in the presence of AdoMet. The use of EcoP151 restriction in presence of sinefungin as an improved tool for serial analysis of gene expression is discussed.
|Item Type:||Journal Article|
|Additional Information:||Copyright for this article belongs to Elsevier.|
|Keywords:||AdoMet;Sinefungin;EcoP15I;DNA topology;Site requirement;SAGE|
|Department/Centre:||Division of Biological Sciences > Biochemistry|
|Date Deposited:||17 Oct 2005|
|Last Modified:||19 Sep 2010 04:20|
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