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Non-viral ex vivo hepatic gene transfer by in situ lipofection of liver and intraperitoneal transplantation of hepatocytes

Rangarajan, PN and Vatsala, PG and Ashok, MS and Srinivas, VK and Habibullah, CM and Padmanaban, G (1997) Non-viral ex vivo hepatic gene transfer by in situ lipofection of liver and intraperitoneal transplantation of hepatocytes. In: Gene, 190 (1). pp. 217-221.

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Official URL: http://dx.doi.org/10.1016/S0378-1119(96)00640-3

Abstract

Perfusion of liver with plasmid DNA-lipofectin complexes via the portal vein results in efficient accumulation of the vector in hepatocytes. Such hepatocytes, when administered intraperitoneally into a hepatectomized rat, repopulate the liver and express the transgene efficiently. This procedure obviates the need for large-scale hepatocyte culture for ex vivo gene transfer. Further, intraperitoneal transplantation is a simple and cost-effective strategy of introducing genetically modified hepatocytes into liver. Thus, in situ lipofection of liver and intraperitoneal transfer of hepatocytes can be developed into a novel method of non-viral ex vivo gene transfer technique that has applications in the treatment of metabolic disorders of liver and hepatic gene therapy.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Elsevier Science.
Keywords: Gene therapy;Fulminant hepatic failure;Ovine growth hormone
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 03 Aug 2011 09:59
Last Modified: 03 Aug 2011 09:59
URI: http://eprints.iisc.ernet.in/id/eprint/38326

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