Rangarajan, PN and Vatsala, PG and Ashok, MS and Srinivas, VK and Habibullah, CM and Padmanaban, G (1997) Non-viral ex vivo hepatic gene transfer by in situ lipofection of liver and intraperitoneal transplantation of hepatocytes. In: Gene, 190 (1). pp. 217-221.
Non-viral_ex_vivo_hepatic_gene.pdf - Published Version
Restricted to Registered users only
Download (637Kb) | Request a copy
Perfusion of liver with plasmid DNA-lipofectin complexes via the portal vein results in efficient accumulation of the vector in hepatocytes. Such hepatocytes, when administered intraperitoneally into a hepatectomized rat, repopulate the liver and express the transgene efficiently. This procedure obviates the need for large-scale hepatocyte culture for ex vivo gene transfer. Further, intraperitoneal transplantation is a simple and cost-effective strategy of introducing genetically modified hepatocytes into liver. Thus, in situ lipofection of liver and intraperitoneal transfer of hepatocytes can be developed into a novel method of non-viral ex vivo gene transfer technique that has applications in the treatment of metabolic disorders of liver and hepatic gene therapy.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to Elsevier Science.|
|Keywords:||Gene therapy;Fulminant hepatic failure;Ovine growth hormone|
|Department/Centre:||Division of Biological Sciences > Biochemistry|
|Date Deposited:||03 Aug 2011 09:59|
|Last Modified:||03 Aug 2011 09:59|
Actions (login required)