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Synergistic effect of UVB radiation and age on HMPS enzymes in rat lens homogenate

Reddy, Bhanuprakash G and Bhat, Seetharam K (1998) Synergistic effect of UVB radiation and age on HMPS enzymes in rat lens homogenate. In: Journal of Photochemistry and Photobiology B: Biology, 43 (1). pp. 56-60.

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Official URL: http://dx.doi.org/10.1016/S1011-1344(98)00082-7

Abstract

The behaviour of rat lenticular enzymes, glucose-6-phosphate dehydrogena.se (G6PD, EC: 1.1.1.49) and 6-phosphogluconate dehydrogenase (6PGD, EC: 1.1.1.44) as a function of age and UVB irradiation (in vitro) was investigated by irradiating the lens homogenate from 3-and 12-month-old rats at 300 nm (100 μW cm 2). In the 3-month-old group the specific activities of G6PD and 6PGD were reduced by 26% and 42%, respectively, after 24 h of irradiation, whereas in the 12-month-old group the decrease was 38% and 49% respectively, which suggests that the susceptibility of HMPS enzymes to UVB damage is higher in older lenses. The decrease in specitic activity was associated with a change in apparent Km and Vmax (marginal in 3 months and significant in 12 months) of these enzymes due to UVB irradiation. UVB irradiation also decreased the levels of NADPH and NADPH/NADP ratio. These changes, altered activities of G6PD and 6PGD and altered levels of NADPH. may in turn have a bearing on lens transparency.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Elsevier Science.
Keywords: Age;Enzymes;Lens;NADPH;Rat;UVB-radiation.
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 12 Jul 2011 07:51
Last Modified: 12 Jul 2011 07:51
URI: http://eprints.iisc.ernet.in/id/eprint/38775

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