Anuradha, S and Tripathi, Pankaj and Mahajan, Kiran and Muniyappa, K (2005) Meiosis-specific yeast Hop1 protein promotes pairing of double-stranded DNA helices via G/C isochores. In: Biochemical and Biophysical Research Communications, 336 (3). pp. 934-941.
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In eukaryotes, genetic exchange between homologs is facilitated by a tripartite proteinaceous structure called the synaptonemal complex(SC). Several lines of evidence indicate that the genes that encode components of SC are essential for meiotic chromosome pairing and recombination. However, the molecular mechanism by which SC proteins promote these processes is obscure. Here, we report that Saccharomyces cerevisiae Hop1 protein, a component of SC, promotes pairing between two double-stranded DNA helices containing a centrally located G/C isochore. Significantly, pairing was rapid and robust, and required four contiguous G/C base pairs. Using a series of truncated DNA double helices we show that 20 bp on either side of 8 bp target G/C sequenceis essential for pairing. To our knowledge, Hop1 is the first protein shown to do so from yeast or any other organism. These results indicate that Hop1 protein is likely to play a direct role in meiotic chromosome pairing and recombination.
|Item Type:||Journal Article|
|Additional Information:||Copyright for this article belongs to Elsevier Inc.|
|Keywords:||Saccharomyces cerevisiae;Hop1 protein;Chromosome synapsis;Recombination;Synaptonemal complex;G/C isochore|
|Department/Centre:||Division of Biological Sciences > Biochemistry|
|Date Deposited:||23 Nov 2007|
|Last Modified:||19 Sep 2010 04:21|
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