Prakash, Divya G and Anish, RV and Jagadeesh, G and Chakravortty, Dipshikha (2011) Bacterial transformation using micro-shock waves. In: Analytical Biochemistry, 419 (2). pp. 292-301.
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Shock waves are one of the most competent mechanisms of energy dissipation observed in nature. We have developed a novel device to generate controlled micro-shock waves using an explosive-coated polymer tube. In this study, we harnessed these controlled micro-shock waves to develop a unique bacterial transformation method. The conditions were optimized for the maximum transformation efficiency in Escherichia coli. The maximum transformation efficiency was obtained when we used a 30 cm length polymer tube, 100 mu m thick metal foil, 200 mM CaCl(2), 1 ng/mu l plasmid DNA concentration, and 1 x 10(9) cell density. The highest transformation efficiency achieved (1 x 10(-5) transformants/cell) was at least 10 times greater than the previously reported ultrasound-mediated transformation (1 x 10(-6) transformants/cell). This method was also successfully employed for the efficient and reproducible transformation of Pseudomonas aeruginosa and Salmonella typhimurium. This novel method of transformation was shown to be as efficient as electroporation with the added advantage of better recovery of cells, reduced cost (40 times cheaper than a commercial electroporator), and growth phase independent transformation. (C) 2011 Elsevier Inc. All rights reserved.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to Elsevier Science.|
|Keywords:||Novel transformation method;Shock wave;Micro-shock wave; Bacterial transformation|
|Department/Centre:||Division of Biological Sciences > Microbiology & Cell Biology
Division of Mechanical Sciences > Aerospace Engineering (Formerly, Aeronautical Engineering)
|Date Deposited:||17 Nov 2011 08:45|
|Last Modified:||17 Nov 2011 08:45|
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