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Direct detection of Salmonella without pre-enrichment in milk, ice-cream and fruit juice by PCR against hilA gene

Marathe, Sandhya A and Chowdhury, Rebecca and Bhattacharya, Rahul and Nagarajan, Arvindhan Govindasamy and Chakravortty, Dipshikha (2012) Direct detection of Salmonella without pre-enrichment in milk, ice-cream and fruit juice by PCR against hilA gene. In: Food Control, 23 (2). pp. 559-563.

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Official URL: http://dx.doi.org/10.1016/j.foodcont.2011.08.005

Abstract

A novel PCR based assay was devised to specifically detect contamination of any Salmonella serovar in milk, fruit juice and ice-cream without pre-enrichment. This method utilizes primers against hilA gene which is conserved in all Salmonella serovars and absent from the close relatives of Salmonella. An optimized protocol, in terms time and money, is provided for the reduction of PCR contaminants from milk, ice-cream and juice through the use of routine laboratory chemicals. The simplicity, efficiency (time taken 3-4 h) and sensitivity (to about 5-10 CFU/ml) of this technique confers a unique advantage over other previously used time consuming detection techniques. This technique does not involve pre-enrichment of the samples or extensive sample processing, which was a pre-requisite in most of the other reported studies. Hence, this assay can be ideal for adoption, after further fine tuning, by food quality control for timely detection of Salmonella contamination as well as other food-borne pathogens (with species specific primers) in food especially milk, ice-cream and fruit juice. (C) 2011 Elsevier Ltd. All rights reserved.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Elsevier Science.
Keywords: Salmonella;PCR;Milk;Ice-cream;Clear and pulpy fruit juice
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 22 Nov 2011 06:29
Last Modified: 22 Nov 2011 06:29
URI: http://eprints.iisc.ernet.in/id/eprint/42271

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