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Crystallization and preliminary X-ray diffraction studies of sortase A from Streptococcus pneumoniae

Misra, Anurag and Biswas, Tora and Das, Sreetama and Marathe, Uttara and Sehgal, Devinder and Roy, Rajendra P and Suryanarayanarao, Ramakumar (2011) Crystallization and preliminary X-ray diffraction studies of sortase A from Streptococcus pneumoniae. In: Acta Crystallographica Section F, 67 (Part 1). pp. 1195-1198.

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Official URL: http://scripts.iucr.org/cgi-bin/paper?S17443091110...

Abstract

Sortases are cell-membrane-anchored cysteine transpeptidases that are essential for the assembly and anchoring of cell-surface adhesins in Gram-positive bacteria. Thus, they play critical roles in virulence, infection and colonization by pathogens. Sortases have been classified into four types based on their primary sequence and the target-protein motifs that they recognize. All Gram-positive bacteria express a class A housekeeping sortase (SrtA). Sortase A from Streptococcus pneumoniae (NP_358691) has been crystallized in two crystal forms. Diamond-shaped crystals of Delta N(59)SrtA diffracted to 4.0 angstrom resolution and belonged to a tetragonal system with unit-cell parameters a = b = 122.8, c = 86.5 angstrom, alpha = beta = gamma = 90 degrees, while rod-shaped crystals of Delta N(81)SrtA diffracted to 2.91 angstrom resolution and belonged to the monoclinic space group P2(1) with unit-cell parameters a = 66.8, b = 103.47, c = 74.79 angstrom, alpha = gamma = 90, beta = 115.65 degrees. The Matthews coefficient (V(M) = 2.77 angstrom(3) Da(-1)) with similar to 56% solvent content suggested the presence of four molecules in the asymmetric unit for Delta N(81)SrtA. Also, a multi-copy search using a monomer as a probe in the molecular-replacement method resulted in the successful location of four sortase molecules in the asymmetric unit, with statistics R = 41.61, R(free) = 46.44, correlation coefficient (CC) = 64.31, CC(free) = 57.67.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to John Wiley & Sons.
Department/Centre: Division of Physical & Mathematical Sciences > Physics
Date Deposited: 07 Dec 2011 07:08
Last Modified: 07 Dec 2011 07:08
URI: http://eprints.iisc.ernet.in/id/eprint/42538

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