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Bufferless lysis of erythrocytes for isolation of hemoglobin using modified cellulose acetate membranes

Vanjari, Siva Rama Krishna and Deepthi, I and Sandeep, K and Amrutur, Bharadwaj and Bhat, Navakanta and Srinivasan, Sampath (2012) Bufferless lysis of erythrocytes for isolation of hemoglobin using modified cellulose acetate membranes. In: Biotechnology and Bioprocess Engineering (BBE), 17 (2). pp. 309-315.

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Abstract

This paper presents a modified cellulose acetate membrane prepared using a dry casting technique that can be used to perform lysis of erythrocytes and isolation of hemoglobin. Isolation of hemoglobin is thus achieved without the use of lysis buffers. Cellulose acetate (CA) membranes are embedded with ammonium chloride (NH4Cl) and potassium bicarbonate (KHCO3), which act as lysing agents. The presence of embedded salts is confirmed using EDS analysis. The pores in the CA membrane act as filters. The average pore size in these membranes is designed to be 1.5 mu M, as characterized by SEM analysis, so that they allow hemoglobin to pass through and block all other cells and unlysed erythrocytes present in blood. When a drop of blood is added to the membrane, the NH4Cl and KHCO3 embedded in the membrane dissolve in plasma and lyse the erythrocytes. The filtered hemoglobin is characterized using UV-Vis Spectroscopy. The results indicate extraction of higher concentration of hemoglobin compared with conventional methods.

Item Type: Journal Article
Additional Information: Copyright of this article is belongs to Korean Society for Biotechnology and Bioengineering.
Keywords: erythrocytes;lysis;bufferless;cellulose acetate membranes; filtration;isolation of hemoglobin
Department/Centre: Division of Chemical Sciences > Inorganic & Physical Chemistry
Division of Electrical Sciences > Electrical Communication Engineering
Other Centres/Units > Centre for Nano Science and Engineering
Date Deposited: 14 Aug 2012 05:32
Last Modified: 14 Aug 2012 05:35
URI: http://eprints.iisc.ernet.in/id/eprint/44494

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