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Regulation of lipid biosynthesis by phosphatidylinositol-specific phospholipase C through the transcriptional repression of upstream activating sequence inositol containing genes

Rupwate, Sunny D and Rupwate, Preeti S and Rajasekharan, Ram (2012) Regulation of lipid biosynthesis by phosphatidylinositol-specific phospholipase C through the transcriptional repression of upstream activating sequence inositol containing genes. In: FEBS LETTERS, 586 (10). pp. 1555-1560.

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Official URL: http://dx.doi.org/10.1016/j.febslet.2012.04.022

Abstract

The regulation of phospholipid biosynthesis in Saccharomyces cerevisiae through cis-acting upstream activating sequence inositol (UAS(ino)) and trans-acting elements, such as the INO2-INO4 complex and OPI1 by inositol supplementation in growth is thoroughly studied. In this study, we provide evidence for the regulation of lipid biosynthesis by phosphatidylinositol-specific phospholipase C (PLC) through UAS(ino) and the trans-acting elements. Gene expression analysis and radiolabelling experiments demonstrated that the overexpression of rice PLC in yeast cells altered phospholipid biosynthesis at the levels of transcriptional and enzyme activity. This is the first report implicating PLC in the direct regulation of lipid biosynthesis. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Item Type: Journal Article
Additional Information: copyright for this article belongs to Elsevier
Keywords: Inositol auxotrophy; Lipid biosynthesis; Phospholipase C; UASino
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 20 Jul 2012 09:25
Last Modified: 20 Jul 2012 09:25
URI: http://eprints.iisc.ernet.in/id/eprint/44651

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