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Sensitive Liquid Chromatography-Tandem Mass Spectrometry Method for the Determination of Olanzapine in Human Urine

Urdigere, Anil Kumar Rangachar and Besagarahally, Bhasakra Laxmaiah and Basavaiah, Kanakapura (2012) Sensitive Liquid Chromatography-Tandem Mass Spectrometry Method for the Determination of Olanzapine in Human Urine. In: ARABIAN JOURNAL FOR SCIENCE AND ENGINEERING, 37 (5). pp. 1381-1387.

Full text not available from this repository.
Official URL: http://dx.doi.org/10.1007/s13369-012-0249-7

Abstract

A sensitive and selective liquid chromatographic-tandem mass spectrometric (LC-MS-MS) method was developed to determine olanzapine (OLZ) in human urine. After solid-phase extraction with SPE cartridge, the urine sample was analysed on a C-18 column (Symmetry 3.5 mu m, 50 x 4.6 mm i.d) interfaced with a triple quadrupole tandem mass spectrometer. Positive electrospray ionization was employed as the ionization source. The mobile phase consisted of ammonium acetate (pH 7.8)-acetonitrile (10:90, v/v). The method was linear over a concentration range of 1-100 ngml(-1). The lower limit of quantitation was 1 ngml(-1). The intra-day and inter-day relative standard deviation across three validation runs over the entire concentration range was < 11.5 %. The accuracy determined at three concentrations (8.0, 50.0 and 85.0 ngml(-1) OLZ) was within +/- 1.21 % in terms of relative errors.

Item Type: Journal Article
Keywords: Olanzapine; Quantification; Mass spectrometry
Department/Centre: Others
Date Deposited: 03 Aug 2012 06:24
Last Modified: 03 Aug 2012 06:24
URI: http://eprints.iisc.ernet.in/id/eprint/44887

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