Urdigere, Anil Kumar Rangachar and Besagarahally, Bhasakra Laxmaiah and Basavaiah, Kanakapura (2012) Sensitive Liquid Chromatography-Tandem Mass Spectrometry Method for the Determination of Olanzapine in Human Urine. In: ARABIAN JOURNAL FOR SCIENCE AND ENGINEERING, 37 (5). pp. 1381-1387.Full text not available from this repository.
A sensitive and selective liquid chromatographic-tandem mass spectrometric (LC-MS-MS) method was developed to determine olanzapine (OLZ) in human urine. After solid-phase extraction with SPE cartridge, the urine sample was analysed on a C-18 column (Symmetry 3.5 mu m, 50 x 4.6 mm i.d) interfaced with a triple quadrupole tandem mass spectrometer. Positive electrospray ionization was employed as the ionization source. The mobile phase consisted of ammonium acetate (pH 7.8)-acetonitrile (10:90, v/v). The method was linear over a concentration range of 1-100 ngml(-1). The lower limit of quantitation was 1 ngml(-1). The intra-day and inter-day relative standard deviation across three validation runs over the entire concentration range was < 11.5 %. The accuracy determined at three concentrations (8.0, 50.0 and 85.0 ngml(-1) OLZ) was within +/- 1.21 % in terms of relative errors.
|Item Type:||Journal Article|
|Keywords:||Olanzapine; Quantification; Mass spectrometry|
|Date Deposited:||03 Aug 2012 06:24|
|Last Modified:||03 Aug 2012 06:24|
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