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Comparative Transcriptomics of H. pylori Strains AM5, SS1 and Their hpyAVIBM Deletion Mutants: Possible Roles of Cytosine Methylation

Kumar, Ritesh and Mukhopadhyay, Asish K and Ghosh, Prachetash and Rao, Desirazu N (2012) Comparative Transcriptomics of H. pylori Strains AM5, SS1 and Their hpyAVIBM Deletion Mutants: Possible Roles of Cytosine Methylation. In: PLOS ONE, 7 (8).

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Official URL: http://dx.doi.org/10.1371/journal.pone.0042303

Abstract

Helicobacter pylori is an important human pathogen and one of the most successful chronic colonizers of the human body. H. pylori uses diverse mechanisms to modulate its interaction with the host in order to promote chronic infection and overcome host immune response. Restriction-modification genes are a major part of strain-specific genes present in H. pylori. The role of N-6 -adenine methylation in bacterial gene regulation and virulence is well established but not much is known about the effect of C-5 -cytosine methylation on gene expression in prokaryotes. In this study, it was observed by microarray analysis and RT-PCR, that deletion of an orphan C-5 -cytosine methyltransferase, hpyAVIBM in H. pylori strains AM5and SS1 has a significant effect on the expression of number of genes belonging to motility, adhesion and virulence. AM Delta DhpyAVIBM mutant strain has a different LPS profile and is able to induce high IL-8 production compared to wild-type. hpyAVIBM from strain 26695 is able to complement mutant SS1 and AM5 strains. This study highlights a possible significance of cytosine methylation in the physiology of H. pylori.

Item Type: Journal Article
Additional Information: Copy right for this article belongs to Public Library Science USA
Keywords: PATHOGEN HELICOBACTER-PYLORI;MEMBRANE PROTEIN EXPRESSION; DNA ADENINE METHYLATION;COMPARATIVE GENOMICS;GASTRIC PATHOGEN;VIRULENCE GENES;MISMATCH REPAIR;PHASE VARIATION; WEST-BENGAL; INFECTION
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 15 Oct 2012 04:43
Last Modified: 15 Oct 2012 04:44
URI: http://eprints.iisc.ernet.in/id/eprint/45091

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