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Expression of VP1 protein of serotype A and O of foot-and-mouth disease virus in transgenic sunnhemp plants and its immunogenicity for guinea pigs

Rao, JP and Agrawal, P and Mohammad, R and Rao, SK and Reddy, GR and Dechamma, HJ and Suryanarayana, VVS (2012) Expression of VP1 protein of serotype A and O of foot-and-mouth disease virus in transgenic sunnhemp plants and its immunogenicity for guinea pigs. In: ACTA VIROLOGICA, 56 (2). pp. 91-99.

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Official URL: http://dx.doi.org/10.4149/av_2012_02_91

Abstract

Recently, transgenic plants expressing immunogenic proteins of foot-and-mouth disease virus (FMDV) have been used as oral or parenteral vaccines against foot-and-mouth disease (FMD). They exhibit advantages like cost effectiveness, absence of processing, thermostability, and easy oral application. FMDV VP1 protein of single serotype has been mostly used as immunogen. Here we report the development of a bivalent vaccine with tandem-linked VP1 proteins of two serotypes, A and O, present in transgenic forage crop Crotalaria juncea. The expression of the bivalent protein in the transgenic plants was confirmed by Western blot analysis. Guinea pig reacted to orally or parenterally applied vaccine by humoral as well as cell-mediated immune responses including serum antibodies and stimulated lymphocytes, respectively. The vaccine protected the animals against a challenge with the virus of serotype A as well as O. This is the first report on the development of a bivalent FMD vaccine using a forage crop.

Item Type: Journal Article
Additional Information: Copyright for this article belongs to the AE Press .
Keywords: foot-and-mouth disease;sunnhemp;Agrobacterium tumefaciens;FMDV-VP1 gene;serotype O and A;in planta transformation;transgenic plants;bivalent vaccine
Department/Centre: Division of Biological Sciences > Centre for Ecological Sciences
Date Deposited: 07 Dec 2012 10:14
Last Modified: 07 Dec 2012 10:14
URI: http://eprints.iisc.ernet.in/id/eprint/45107

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