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Point Spread Function Engineering for Super-Resolution Single-Photon and Multiphoton Fluorescence Microscopy

Mondal, Partha Pratim and Diaspro, Alberto (2012) Point Spread Function Engineering for Super-Resolution Single-Photon and Multiphoton Fluorescence Microscopy. [Book Chapter]

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Official URL: http://dx.doi.org/10.1016/B978-0-12-407670-9.00004...

Abstract

Super-resolution imaging techniques are of paramount interest for applications in bioimaging and fluorescence microscopy. Recent advances in bioimaging demand application-tailored point spread functions. Here, we present some approaches for generating application-tailored point spread functions along with fast imaging capabilities. Aperture engineering techniques provide interesting solutions for obtaining desired system point spread functions. Specially designed spatial filters—realized by optical mask—are outlined both in a single-lens and 4Pi configuration. Applications include depth imaging, multifocal imaging, and super-resolution imaging. Such an approach is suitable for fruitful integration with most existing state-of-art imaging microscopy modalities.

Item Type: Book Chapter
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Keywords: Super-resolution Microscopy;Multiple Excitation Spot Optical Microscopy;Point Spread Function Engineering;Multiphoton Microscopy;4Pi-geometryAperture Engineering
Department/Centre: Division of Physical & Mathematical Sciences > Instrumentation and Applied Physics (Formally ISU)
Date Deposited: 01 Apr 2013 11:46
Last Modified: 01 Apr 2013 11:46
URI: http://eprints.iisc.ernet.in/id/eprint/46253

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