Gel Purified Lipl32: A Prospective Antigen for Detection of Leptospirosis.

Tahiliani, P and Kumar, Mohan M and Chandu, D and Kumar, A and Nagaraj, C and Nandi, D (2005) Gel Purified Lipl32: A Prospective Antigen for Detection of Leptospirosis. In: Journal of Postgraduate Medicine, 51 (3). pp. 164-168.

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Abstract

Background: Leptospirosis, a zoonosis, is a re-emerging disease, affecting populations across the globe. However, the current methods of diagnosis are time- consuming, cumbersome, imprecise or expensive. Aim: To develop an assay for differential and early diagnosis of Leptospirosis. Methods and Material: IgG based ELISA for evaluation of three antigens, namely, a gel-purified recombinant protein (rLipL32), secreted proteins and whole organism sonicates of Leptospira spp. The antigens were evaluated using, rabbit polyclonal antiserum and human sera samples. Results: Studies with a rabbit polyclonal antiserum indicated the utility of these antigens in differentiating Leptospira from other common pathogenic organisms. Evaluation of these antigens with fifteen representative human serum samples indicated gel-purified rLipL32 to be a potentially useful antigen for detection of leptospirosis. The results obtained with IgG ELISA were correlated with the results of microscopic agglutination test (MAT). Conclusion: Gel-purified rLipL32 is a valuable antigen for early and accurate diagnosis of leptospirosis. Further evaluation of this assay in field conditions and larger sera samples will indicate its suitability in case of an epidemic.

Item Type:	Journal Article
Additional Information:	The copyright belongs to Medknow Publications
Keywords:	Leptospira;LipL32;Human IgG ELISA;Gel elution
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	04 Jan 2006
Last Modified:	19 Sep 2010 04:22
URI:	http://eprints.iisc.ernet.in/id/eprint/4843

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