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In vitro Evidence that an Aqueous Extract of Centella asiatica Modulates alpha-Synuclein Aggregation Dynamics

Berrocal, Ruben and Vasudevaraju, Padmaraju and Indi, Shantinath Satappa and Rao, Krothapalli Raja Surya Sambasiva and Rao, KS (2014) In vitro Evidence that an Aqueous Extract of Centella asiatica Modulates alpha-Synuclein Aggregation Dynamics. In: JOURNAL OF ALZHEIMERS DISEASE, 39 (2). pp. 457-465.

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Official URL: http://dx.doi.org/10.3233/JAD-131187

Abstract

alpha-Synuclein aggregation is one of the major etiological factors implicated in Parkinson's disease (PD). The prevention of aggregation of alpha-synuclein is a potential therapeutic intervention for preventing PD. The discovery of natural products as alternative drugs to treat PD and related disorders is a current trend. The aqueous extract of Centella asiatica (CA) is traditionally used as a brain tonic and CA is known to improve cognition and memory. There are limited data on the role of CA in modulating amyloid-beta (A beta) levels in the brain and in A beta aggregation. Our study focuses on CA as a modulator of the alpha-synuclein aggregation pattern in vitro. Our investigation is focused on: (i) whether the CA leaf aqueous extract prevents the formation of aggregates from monomers (Phase I: alpha-synuclein + extract co-incubation); (ii) whether the CA aqueous extract prevents the formation of fibrils from oligomers (Phase II: extract added after oligomers formation); and (iii) whether the CA aqueous extract disintegrates the pre-formed fibrils (Phase III: extract added to mature fibrils and incubated for 9 days). The aggregation kinetics are studied using a thioflavin-T assay, circular dichroism, and transmission electron microscopy. The results showed that the CA aqueous extract completely inhibited the alpha-synuclein aggregation from monomers. Further, CA extract significantly inhibited the formation of oligomer to aggregates and favored the disintegration of the preformed fibrils. The study provides an insight in finding new natural products for future PD therapeutics.

Item Type: Journal Article
Additional Information: Copyright for this article belongs to the IOS PRESS, NETHERLANDS
Keywords: alpha-Synuclein; anti-aggregation; Centella asiatica; circular dichroism; natural products; Parkinson's disease; transmission electron microscopy
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Depositing User: Id for Latest eprints
Date Deposited: 13 Mar 2014 07:19
Last Modified: 13 Mar 2014 07:19
URI: http://eprints.iisc.ac.in/id/eprint/48560

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