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Lactoylglutathione lyase, a critical enzyme in methylglyoxal detoxification, contributes to survival of Salmonella in the nutrient rich environment

Chakraborty, Sangeeta and Gogoi, Mayuri and Chakravortty, Dipshikha (2015) Lactoylglutathione lyase, a critical enzyme in methylglyoxal detoxification, contributes to survival of Salmonella in the nutrient rich environment. In: VIRULENCE, 6 (1). pp. 50-65.

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Official URL: http://dx.doi.org/10.4161/21505594.2014.983791

Abstract

Glyoxalase I which is synonymously known as lactoylglutathione lyase is a critical enzyme in methylglyoxal (MG) detoxification. We assessed the STM3117 encoded lactoylglutathione lyase (Lgl) of Salmonella Typhimurium, which is known to function as a virulence factor, due in part to its ability to detoxify methylglyoxal. We found that STM3117 encoded Lgl isomerises the hemithioacetal adduct of MG and glutathione (GSH) into S-lactoylglutathione. Lgl was observed to be an outer membrane bound protein with maximum expression at the exponential growth phase. The deletion mutant of S. Typhimurium (lgl) exhibited a notable growth inhibition coupled with oxidative DNA damage and membrane disruptions, in accordance with the growth arrest phenomenon associated with typical glyoxalase I deletion. However, growth in glucose minimal medium did not result in any inhibition. Endogenous expression of recombinant Lgl in serovar Typhi led to an increased resistance and growth in presence of external MG. Being a metalloprotein, Lgl was found to get activated maximally by Co2+ ion followed by Ni2+, while Zn2+ did not activate the enzyme and this could be attributed to the geometry of the particular protein-metal complex attained in the catalytically active state. Our results offer an insight on the pivotal role of the virulence associated and horizontally acquired STM3117 gene in non-typhoidal serovars with direct correlation of its activity in lending survival advantage to Salmonella spp.

Item Type: Journal Article
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Additional Information: Copy right for this article belongs to the TAYLOR & FRANCIS INC, 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA
Keywords: ESCHERICHIA-COLI K-12; GLYOXALASE-I; THREONINE METABOLISM; STREPTOCOCCUS-MUTANS; SEROVAR TYPHIMURIUM; BACILLUS-SUBTILIS; YERSINIA-PESTIS; DNA-DAMAGE; CELL-DEATH; IDENTIFICATION
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 20 Apr 2015 07:46
Last Modified: 20 Apr 2015 07:46
URI: http://eprints.iisc.ernet.in/id/eprint/51268

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