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Successful treatment of biofilm infections using shock waves combined with antibiotic therapy

Gnanadhas, Divya Prakash and Elango, Monalisha and Janardhanraj, S and Srinandan, CS and Datey, Akshay and Strugnell, Richard A and Gopalan, Jagadeesh and Chakravortty, Dipshikha (2015) Successful treatment of biofilm infections using shock waves combined with antibiotic therapy. In: SCIENTIFIC REPORTS, 5 .

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Official URL: http://dx.doi.org/10.1038/srep17440

Abstract

Many bacteria secrete a highly hydrated framework of extracellular polymer matrix on suitable substrates and embed within the matrix to form a biofilm. Bacterial biofilms are observed on many medical devices, endocarditis, periodontitis and lung infections in cystic fibrosis patients. Bacteria in biofilm are protected from antibiotics and >1,000 times of the minimum inhibitory concentration may be required to treat biofilm infections. Here, we demonstrated that shock waves could be used to remove Salmonella, Pseudomonas and Staphylococcus biofilms in urinary catheters. The studies were extended to a Pseudomonas chronic pneumonia lung infection and Staphylococcus skin suture infection model in mice. The biofilm infections in mice, treated with shock waves became susceptible to antibiotics, unlike untreated biofilms. Mice exposed to shock waves responded to ciprofloxacin treatment, while ciprofloxacin alone was ineffective in treating the infection. These results demonstrate for the first time that, shock waves, combined with antibiotic treatment can be used to treat biofilm infection on medical devices as well as in situ infections.

Item Type: Journal Article
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Additional Information: Copy right for this article belongs to the NATURE PUBLISHING GROUP, MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Division of Mechanical Sciences > Aerospace Engineering (Formerly, Aeronautical Engineering)
Others
Date Deposited: 07 Jan 2016 07:22
Last Modified: 07 Jan 2016 07:22
URI: http://eprints.iisc.ernet.in/id/eprint/53038

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