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Intracellular delivery of antibodies by chimeric Sesbania mosaic virus (SeMV) virus like particles

Abraham, Ambily and Natraj, Usha and Karande, Anjali A and Gulati, Ashutosh and Murthy, Mathur RN and Murugesan, Sathyabalan and Mukunda, Pavithra and Savithri, Handanahal S (2016) Intracellular delivery of antibodies by chimeric Sesbania mosaic virus (SeMV) virus like particles. In: SCIENTIFIC REPORTS, 6 .

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Official URL: http://dx.doi.org/10.1038/srep21803

Abstract

The therapeutic potential of antibodies has not been fully exploited as they fail to cross cell membrane. In this article, we have tested the possibility of using plant virus based nanoparticles for intracellular delivery of antibodies. For this purpose, Sesbania mosaic virus coat protein (CP) was genetically engineered with the B domain of Staphylococcus aureus protein A (SpA) at the beta H-beta I loop, to generate SeMV loop B (SLB), which self-assembled to virus like particles (VLPs) with 43 times higher affinity towards antibodies. CP and SLB could internalize into various types of mammalian cells and SLB could efficiently deliver three different monoclonal antibodies-D6F10 (targeting abrin), anti-a-tubulin (targeting intracellular tubulin) and Herclon (against HER2 receptor) inside the cells. Such a mode of delivery was much more effective than antibodies alone treatment. These results highlight the potential of SLB as a universal nanocarrier for intracellular delivery of antibodies.

Item Type: Journal Article
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Additional Information: Copy right for this article belongs to the NATURE PUBLISHING GROUP, MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
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Date Deposited: 02 Apr 2016 07:45
Last Modified: 02 Apr 2016 07:45
URI: http://eprints.iisc.ernet.in/id/eprint/53548

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