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A novel viral RNA helicase with an independent translation enhancement activity

Abraham, Ambily and Savithri, Handanahal S (2016) A novel viral RNA helicase with an independent translation enhancement activity. In: FEBS LETTERS, 590 (8). pp. 1187-1199.

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Official URL: http://dx.doi.org/10.1002/1873-3468.12145

Abstract

RNA helicases have not been identified among negative sense RNA viruses. In this study, it is shown that Nonstructural protein (NSs) of Groundnut bud necrosis virus (GBNV) acts as a Mg2+- and ATP-dependent bipolar RNA helicase. Biophysical and biochemical analysis of the deletion mutants (N Delta 124 NSs, C Delta 80 NSs) revealed that both the N- and C-terminal residues are required for substrate binding, oligomerization and helicase activity, but are dispensable for ATPase activity. Interestingly, NSs could enhance the translation of RNA (similar to 10-fold) independent of its helicase activity. This is the first report of a RNA helicase from negative strand RNA viruses.

Item Type: Journal Article
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Additional Information: Copy right for this article belongs to the WILEY-BLACKWELL, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
Keywords: bipolar RNA helicase; Groundnut bud necrosis virus; nonstructural protein; translation enhancer
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 11 Jun 2016 07:52
Last Modified: 11 Jun 2016 07:52
URI: http://eprints.iisc.ernet.in/id/eprint/53906

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