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Optical Diagnostics of Osteoblast Cells and Osteogenic Drug Screening

Kolanthai, Elayaraj A and Veerla, Sarath Chandra and Khajuria, Deepak Kumar and Mahapatra, Roy D (2016) Optical Diagnostics of Osteoblast Cells and Osteogenic Drug Screening. In: Conference on Photonic Therapeutics and Diagnostics XII, FEB 13-14, 2016, San Francisco, CA.

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Official URL: http://dx.doi.org/10.1117/12.2217943

Abstract

Microfluidic device based diagnostics involving optical fibre path, in situ imaging and spectroscopy are gaining importance due to recent advances in diagnostics instrumentation and methods, besides other factors such as low amount of reagent required for analysis, short investigation times, and potential possibilities to replace animal model based study in near future. It is possible to grow and monitor tissues in vitro in microfluidic lab-on-chip. It may become a transformative way of studying how cells interact with drugs, pathogens and biomaterials in physiologically relevant microenvironments. To a large extent, progress in developing clinically viable solutions has been constrained because of (i) contradiction between in vitro and in vivo results and (ii) animal model based and clinical studies which is very expensive. Our study here aims to evaluate the usefulness of microfluidic device based 3D tissue growth and monitoring approach to better emulate physiologically and clinically relevant microenvironments in comparison to conventional in vitro 2D culture. Moreover, the microfluidic methodology permits precise high-throughput investigations through real-time imaging while using very small amounts of reagents and cells. In the present study, we report on the details of an osteoblast cell based 3D microfluidic platform which we employ for osteogenic drug screening. The drug formulation is functionalized with fluorescence and other biomarkers for imaging and spectroscopy, respectively. Optical fibre coupled paths are used to obtain insight regarding the role of stress/flow pressure fluctuation and nanoparticle-drug concentration on the osteoblast growth and osteogenic properties of bone.

Item Type: Conference Proceedings
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Additional Information: Copy right for this article belongs to the SPIE-INT SOC OPTICAL ENGINEERING, 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA
Keywords: Osteoblast cells; microfluidic; drug screening; scaffold; tissue engineering; spectroscopy; fluorescence imaging
Department/Centre: Division of Mechanical Sciences > Aerospace Engineering (Formerly, Aeronautical Engineering)
Date Deposited: 08 Jul 2016 05:57
Last Modified: 08 Jul 2016 05:57
URI: http://eprints.iisc.ernet.in/id/eprint/54167

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