Srivastava, Shireesh and Madras, Giridhar (2001) Hydrolysis of p-nitrophenyl laurate in supercritical carbon dioxide: comparison of two different enzymes. In: Journal of Chemical Technology & Biotechnology, 76 (8). pp. 890-892.
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The enzymatic hydrolysis of p-nitrophenyl laurate (PNPL) to p-nitrophenol (PNP) was studied in supercritical carbon dioxide using crude Hog Pancreas Lipase (HPL) and P roqueforti Lipase (PRL). The two enzymes were compared for their optimal activity temperatures and reaction kinetics. HPL had a higher optimal temperature $(65^oC)$ and better yield (22.5%) compared with PRL whose optimal temperature was $50^oC$ with a 15.3% yield. This is considerably higher than the optimum temperature of $37^oC$ and yields of less than 1% observed in the aqueous medium.
|Item Type:||Journal Article|
|Additional Information:||The copyright belongs to Society of Chemical Industry|
|Keywords:||enzyme catalysed hydrolysis;Hog pancreas lipase;P roqueforti;Supercritical carbon dioxide|
|Department/Centre:||Division of Mechanical Sciences > Chemical Engineering|
|Date Deposited:||20 Mar 2006|
|Last Modified:||19 Sep 2010 04:24|
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