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Hydrolysis of p-nitrophenyl laurate in supercritical carbon dioxide: comparison of two different enzymes

Srivastava, Shireesh and Madras, Giridhar (2001) Hydrolysis of p-nitrophenyl laurate in supercritical carbon dioxide: comparison of two different enzymes. In: Journal of Chemical Technology & Biotechnology, 76 (8). pp. 890-892.

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Abstract

The enzymatic hydrolysis of p-nitrophenyl laurate (PNPL) to p-nitrophenol (PNP) was studied in supercritical carbon dioxide using crude Hog Pancreas Lipase (HPL) and P roqueforti Lipase (PRL). The two enzymes were compared for their optimal activity temperatures and reaction kinetics. HPL had a higher optimal temperature $(65^oC)$ and better yield (22.5%) compared with PRL whose optimal temperature was $50^oC$ with a 15.3% yield. This is considerably higher than the optimum temperature of $37^oC$ and yields of less than 1% observed in the aqueous medium.

Item Type: Journal Article
Additional Information: The copyright belongs to Society of Chemical Industry
Keywords: enzyme catalysed hydrolysis;Hog pancreas lipase;P roqueforti;Supercritical carbon dioxide
Department/Centre: Division of Mechanical Sciences > Chemical Engineering
Date Deposited: 20 Mar 2006
Last Modified: 19 Sep 2010 04:24
URI: http://eprints.iisc.ernet.in/id/eprint/5610

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