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Contryphan Genes and Mature Peptides in the Venom of Nine Cone Snail Species by Transcriptomic and Mass Spectrometric Analysis

Vijayasarathy, Marimuthu and Basheer, Soorej M and Franklin, Jayaseelan Benjamin and Balaram, Padmanabhan (2017) Contryphan Genes and Mature Peptides in the Venom of Nine Cone Snail Species by Transcriptomic and Mass Spectrometric Analysis. In: JOURNAL OF PROTEOME RESEARCH, 16 (2). pp. 763-772.

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Official URL: http://dx.doi.org/10.1021/acs.jproteome.6b00776

Abstract

The occurrence of contryphans, a class of single disulfide-bond-containing peptides, is demonstrated by the analysis of the venom of nine species of cone snails. Ten full gene sequences and two partial gene sequences coding for contryphan precursor proteins have been identified by next-generation sequencing and compared with available sequences. The occurrence of mature peptides in isolated venom has been demonstrated by LC-ESI-MS/MS analysis. De novo sequencing of reduced, alkylated contryphans from C. frigidus and C. araneosus provides evidence of sequence variation and post-translational modification, notably gamma carboxylation of glutamic acid. The characterization of Fr965 (C. frigidus) provides a rare example of a sequence lacking Pro at position 5 in the disulfide loop. The widespread occurrence of contryphan genes and mature peptides in the venom of contyr genes and mature peptidesin the venom of diverse cone snails is suggestive of their potential biological significance.

Item Type: Journal Article
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Additional Information: Copy right for this article belongs to the AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 21 Mar 2017 09:23
Last Modified: 21 Mar 2017 09:23
URI: http://eprints.iisc.ernet.in/id/eprint/56406

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