Anand, B and Gowri, VS and Srinivasan, N (2005) Use of multiple profiles corresponding to a sequence alignment enables effective detection of remote homologues. In: Bioinformatics, 21 (12). pp. 2821-2826.Full text not available from this repository. (Request a copy)
MOTIVATION: Position specific scoring matrices (PSSMs) corresponding to aligned sequences of homologous proteins are commonly used in homology detection. A PSSM is generated on the basis of one of the homologues as a reference sequence, which is the query in the case of PSI-BLAST searches. The reference sequence is chosen arbitrarily while generating PSSMs for reverse BLAST searches. In this work we demonstrate that the use of multiple PSSMs corresponding to a given alignment and variable reference sequences is more effective than using traditional single PSSMs and hidden Markov models. RESULTS: Searches for proteins with known 3-D structures have been made against three databases of protein family profiles corresponding to known structures: (1) One PSSM per family; (2) multiple PSSMs corresponding to an alignment and variable reference sequences for every family; and (3) hidden Markov models. A comparison of the performances of these three approaches suggests that the use of multiple PSSMs is most effective.
|Item Type:||Journal Article|
|Additional Information:||The Copyright belongs to Oxford University Press.|
|Department/Centre:||Division of Biological Sciences > Molecular Biophysics Unit|
|Date Deposited:||01 Mar 2006|
|Last Modified:||27 Aug 2008 11:46|
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