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Distortion of the Ligand Molecule as a Strategy for Modulating Binding Affinity: Further Studies Involving Complexes of Jacalin with beta-Substituted Disaccharides

Abhinav, K V and Sharma, Kaushal and Surolia, A and Vijayan, Mamannamana (2017) Distortion of the Ligand Molecule as a Strategy for Modulating Binding Affinity: Further Studies Involving Complexes of Jacalin with beta-Substituted Disaccharides. In: IUBMB LIFE, 69 (2). pp. 72-78.

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Official URL: http://dx.doi.org/10.1002/iub.1593

Abstract

Crystal structures of jacalin in complex with GlcNAc beta-(1,3) Gal-beta-OMe and Gal beta-(1,3) Gal-beta-OMe have been determined. The binding of the ligands to jacalin is similar to that of analogous -substituted disaccharides. However, the beta-substituted beta-(1,3) linked disaccharides get distorted at the anomeric center and the glycosidic linkage. The distortion results in higher internal energies of the ligands leading to lower affinity to the lectin. This confirms the possibility of using ligand distortion as a strategy for modulating binding affinity. Unlike in the case of alpha-substituted monosaccharides bound to jacalin, where a larger distortion at the anomeric center was observed, smaller distortions are distributed among two centers in the structures of the two beta-substituted beta-(1,3) linked disaccharides presented here. These disaccharides, like the unsubstituted and alpha-substituted counterparts, bind jacalin with the reducing Gal at the primary binding site, indicating that the lower binding affinity of beta-substituted disaccharides is not enough to overcome the intrinsic propensity of Gal -(1,3) Gal-based disaccharides to bind jacalin with the reducing sugar at the primary site. (C) 2017 IUBMB Life, 69(2): 72-78, 2017.

Item Type: Journal Article
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Additional Information: Copy right for this article belongs to the WILEY-BLACKWELL, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 03 Apr 2017 04:37
Last Modified: 03 Apr 2017 04:37
URI: http://eprints.iisc.ernet.in/id/eprint/56438

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