Raha, Tamal and Kaushik, Rajnish and Shaila, MS (2004) Phosphoprotein P of Rinderpest virus binds to plus sense leader RNA: regulation by phosphorylation. In: Virus Research, 104 (2). pp. 191-200.
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The negative sense genome RNA of Rinderpest virus, a Paramyxoviridae, is encapsidated with the nucleocapsid protein N and serves as a template for the viral RNA dependent RNA polymerase for transcription and replication. The viral RNA polymerase consists of the large protein L and the phosphoprotein P functioning as the P–L complex. We provide in this report, evidences for specific binding of P protein of Rinderpest virus to the plus sense leader RNA depending on its phosphorylation status. We have also demonstrated that P protein is released from the le RNA:P protein complex upon phosphorylation in vitro. Finally, we have identified that the C-terminal 358–389 amino acid residues of P protein is involved in le RNA binding. The leader RNA binding may signify a hitherto unidentified role for P protein in the viral RNA synthesis. Moreover, our results indicate a possible role for P protein in the transcription-replication switch through leader RNA binding.
|Item Type:||Journal Article|
|Additional Information:||The copyright belongs to Elsevier.|
|Keywords:||Rinderpest virus;Phosphoprotein;Leader RNA;Replication and transcription|
|Department/Centre:||Division of Biological Sciences > Microbiology & Cell Biology|
|Date Deposited:||24 Apr 2006|
|Last Modified:||19 Sep 2010 04:25|
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