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Conserved amino acids 193–324 of non-structural protein 3 are a dominant source of peptide determinants for $CD4^+$ and $CD8^+$ T cells in a healthy Japanese encephalitis virus-endemic cohort

Kumar, Priti and Sulochana, Paramadevanapalli and Nirmala, Gejjehalli and Haridattatreya, Maganti and Satchidanandam, Vijaya (2004) Conserved amino acids 193–324 of non-structural protein 3 are a dominant source of peptide determinants for $CD4^+$ and $CD8^+$ T cells in a healthy Japanese encephalitis virus-endemic cohort. In: Journal of General Virology, 85 (5). pp. 1131-1143.

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Abstract

Our earlier identification of the non-structural protein 3 (NS3) of Japanese encephalitis virus (JEV) as a dominant $CD4^+$ as well as $CD8^+$ T cell-eliciting antigen in a healthy JEV-endemic cohort with a wide HLA distribution implied the presence of several epitopes dispersed over the length of the protein. Use of various truncated versions of NS3 in lymphocyte stimulation and interferon (IFN)-$\gamma$ secretion assays revealed that amino acids (aa) 193–324 ofNS3were comparablewith, if not superior to, the full-length protein in evoking Th1 responses. The potential of this 14.4 kDa stretch to stimulate IFN-$\gamma$ production from both subtypes of T cells in a manner qualitatively and quantitatively similar to the 68 kDa parent protein suggested the presence within it of both class I and II epitopes and demonstrated that the entire immunogenicity of NS3 was focused on aa 193–324. Interestingly, this segment contained five of the eight helicase motifs of NS3. Analysis of variability of the NS3 protein sequence across 16 JEV isolates revealed complete identity of aa 219–318, which is contained within the above segment, suggesting that NS3-specific epitopes tend to cluster in relatively conserved regions that harbour functionally critical domains of the protein.

Item Type: Journal Article
Additional Information: The copyright belongs to Society for General Microbiology.
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 06 May 2006
Last Modified: 19 Sep 2010 04:26
URI: http://eprints.iisc.ernet.in/id/eprint/6596

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