Sistla, Srivani and Krishnamurthy, Vinita and Rao, Desirazu N (2004) Single-stranded DNA binding and methylation by EcoP1I DNA methyltransferase. In: Biochemical and Biophysical Research Communications, 314 (1). pp. 159-165.
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EcoP1I methyltransferase (M.EcoP1I) belongs to the type III restriction–modification system encoded by prophage P1 that infects Escherichia coli. Binding of M.EcoP1I to double-stranded DNA and single-stranded DNA has been characterized. Binding to both single- and double-stranded DNA could be competed out by unlabeled single-stranded DNA. Metal ions did not influence DNA binding. Interestingly, M.EcoP1I was able to methylate single-stranded DNA. Kinetic parameters were determined for singleand double-stranded DNA methylation. This feature of the enzyme probably functions in protecting the phage genome from restriction by type III restriction enzymes and thus could be considered as an anti-restriction system. This study describing in vitro methylation of single-stranded DNA by the type III methyltransferase EcoP1I allows understanding of the mechanism of action of these enzymes and also their role in the biology of single-stranded phages.
|Item Type:||Journal Article|
|Additional Information:||The copyright belongs to Elsevier.|
|Department/Centre:||Division of Biological Sciences > Biochemistry|
|Date Deposited:||31 May 2006|
|Last Modified:||19 Sep 2010 04:28|
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