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15-Deoxyspergualin modulates Plasmodium falciparum heat shock protein function

Ramya, TNC and Surolia, Namita and Surolia, Avadhesha (2006) 15-Deoxyspergualin modulates Plasmodium falciparum heat shock protein function. In: Biochemical and Biophysical Research Communications, 348 (2). pp. 585-592.

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Abstract

Heat shock proteins are essential for the survival of all cells. The C-terminal EEVD motif of Hsp70 has previously been implicated in binding 15-deoxyspergualin (DSG), an immunosuppressant with antimalarial activity whose mechanism of action is uncertain. We report the cloning, overexpression, and characterization of three members of the heat shock family, PfHsp70-1 (an Hsp70 protein with a C-terminal EEVD motif), PfHsp70-2 (an Hsp70 protein without the EEVD motif), and PfHsp70 interacting protein. The chaperone activity of PfHsp70-1, and PfHsp70-2 was enhanced by ATP and by PfHip. Interestingly, while binding of protein substrates to PfHsp70-1, PfHsp70-2 and PfHip was unaffected in the presence of DSG, the ATP enhanced chaperone activity of PfHsp70-1 but not PfHsp70-2 was stimulated further by DSG. Our finding suggests that the binding partner of DSG in the parasite cellular milieu is PfHsp70-1 and paves the way for the elucidation of the mechanism of antimalarial action of DSG.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Elsevier.
Keywords: Hsp70;Hsp70 interacting protein;Plasmodium falciparum;Chaperone activity;15-Deoxyspergualin
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 27 Nov 2007
Last Modified: 19 Sep 2010 04:32
URI: http://eprints.iisc.ernet.in/id/eprint/8790

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